In the acquire tab, choose a saved or preset laser beam setting in the leica. The leica confocal microscope system b46 weill hall revised65 startup turn on 3 red buttons on far right. Leica sp5 confocal microscope department of molecular biology. Fast frame rates, best resolution and depth imaging with full multichannel capabilities in close cooperation with leading research scientists. Leica tcs sp5 confocal microscopes products home leica leica microsystems. Leica sp5 laser scanning confocal microscope training. Leica tcs sp5 ii acoustooptical beam splitter products. A spinning disk confocal microscope differs slightly from a lscm in how it forms the image.
The white light laserbased sp5x system is a significant advance in confocal microscopy. Instructions for the leica sp5 ii laser scanning confocal microscope. Microscope imaging software products leica microsystems. How can i add the scale bar to an image acquired with leica sp5 confocal microscope using imagej. This new broadband confocal microscope system perfectly merges two worlds in one. Usermanual for leica sp8 confocal universitetet i bergen. The system provides the full range of scan speeds at the highest resolution. Leica las af lite is a platform designed for advanced life science research with leica microsystems confocal and widefield systems. Imaging diverse fluorophores in fixed or live samples. The leica tcs sp5 mp covers a wide range of imaging applications multiphoton and one photon by combining two technologies in one system.
Instead of using mirrors to raster a single laser beam across the. Switch speed in an instant the new leica tcs sp5 ii comes with a tandem. Leica dm6000 cfs upright multiphoton microscope equipped with a tcs sp5 mp laser confocal with both galvonomic and resonance scanners and with spectral hyd detectors. Science lab, the knowledge portal of leica microsystems, offers scientific research and teaching material on the subjects of microscopy. National chiao tung university, bio photonics lab, bypraveen. Page 1 queens medical research institute college for medicine and veterinary medicine university of edinburgh manual for the leica sp5c spectral confocal laser scanning microscope version 1. Leica sp5 laser scanning confocal microscope system. How can i add the scale bar to an image acquired with. Leica true confocal scanningspectral photometric 5.
Leica sp5 inverted confocal duke light microscopy core. Microscopy core facility microscopy service center. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes. The leica tcs sp5 is supplied with the latest version of the licensed leica application suite advanced fluorescence. Leica sp5 ii laser scanning confocal microscope biological. Hexagonal packing of nuclei red by microtubules green in coeonocytic female gametophyte of ginkgo biloba. Confocal microscope system with a broad range of scan speeds suited for highresolution. Leica sp5 tile scan protocol 1 open the las af software lower stage completely. Leica dmi6000cs inverted live cell chamber ludin cube metal halide fluorescence source motorized scanning stage tandem scanners select at software initialization conventional.
Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix checkin and start up. Check under configuration that sp5 is selected if not go to configuration and change this to sp5 press ok the confocal. The basic software allows you to capture images from the. Leica sp5 laser scanning confocal microscope system listing. To maintain information on the most current level, the description of software functions. The leica sp5 laser scanning confocal system is used for advanced multichannel fluorescence and livecell applications. Introduction to confocal laser scanning microscopy leica. The leica sp5 is a ultrahigh speed inverted confocal microscope, featuring 5 simultaneous channels, each driven by any of 7 separate laser beams. The leica sp5 is a pointscan confocal microscope with laser lines at 405, 458, 476, 488, 496, 514, 543, 594, and 633nm. This system has five lasers generating nine lines for fluorescence excitation 405.
The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. With an intuitive user interface and straightforward navigation, it guides the user through any workflow, whether fast image acquisition or sophisticated expert analysis. After the software begins to load youll see text scrolling in the las af start up. The facility is equipped with a leica tsc sp5 upright confocal microscope with conventional and resonant scanner, a nikon eclipse te2000e inverted widefield microscope, a zeiss axio scope. This system has a tandem scanner that has both a standard scanner lower speed, resolution up to 8k x 8k and a resonant scanner higher speed. Quick operation manual for leica sp5 confocalmultiphoton. Fan on turn laser power level to lowest setting min turn argon. Open the leica confocal las af software it takes a few. This confocal is reserved for primary and joint cdb faculty and their personnel. Microscope imaging software microscope imaging software from leica microsystems combines microscope, digital camera and accessories into one fully integrated solution. The program offers full control over the microscope. Leica sp5 laser scanning confocal microscope ucsf drc. Leica sp5 x confocalspectral imaging microscopy system with white light laser. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented.
Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan. Leica tcs sp5 user manual indiana university bloomington. How to use leica confocal microscope sp5 external laser. Leica tcs sp5 confocal louisiana state university health.
The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed. The leica sp5 confocal has a filterfree spectral detector system with five individually tunable channels. With its proven, highefficiency sp detection five channels simultaneously. Introduction to confocal laser scanning microscopy leica this presentation has been put together as a common effort of urs ziegler, anne greet bittermann, mathias hoechli. Leica sp5 inverted confocal microscope with aresonant scanner allowing highspeed confocal imaging and is well suited for livecell imaging and highresolution morphological imaging. Laser point scan confocal with standard and highspeed resonance scanning. I found out recently through a similar situation that if you have an las installer, the software will work even without a software keydongle. Leica application suite x las x is the one software platform for all leica microscopes. Leica tcs sp5 confocal laser scanning microscope user guide. Leica sp5 confocal microscope four laser uv, argon, dpss, hene. Select the objective you want to use either inside software or on microscope ctrl panel do not fysically.
Leica sp5 inverted confocal manual duke light microscopy. When asked to initialize the stage, select 2 focus your sample and locate the field of. Open source free software for a leica tcs sp5 confocal. Rmbf leica sp5 aobs spectral confocal core facilities. Fluorescence lifetime imaging was performed on the penn vet imaging cores leica sp5 ii inverted confocalflim microscope with a 63x water immersion lens by scanning the sample with a 405 nm. The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent. This video shows how to configure the internal laser power wavelength etc. Our leica sp5 ii lscm offers an inverted configuration microscope with automatic focus control outfitted with the following objectives. This manual is the first section of a three part leica tcs sp5 user guide.
Leica sp5 tile scan protocol icahn school of medicine. The confocal system is equipped with multiplel aser lines, including a 405 diode, an argon laser 458, 476, 488, 496,514nm and a white light laser 470670nm in 1 nm increments. After a short moment you will get the leica application suite start screen. What should the user of the laser scanning microscope observe. This 45 minute video is the basic training of the use of the leica sp5 confocal microscope in the mbf. Scanning with confocal using the leica tcs sp5 confocal microscope. How to use leica confocal microscope sp5 external laser part1. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems. The leica tcs sp5 is supplied with the latest version of the licensed. This is a very flexible and fast confocal system build with a leica dmi 6000 cs inverted, fully automated microscope with motorized. Leica tcs sp5 confocal this is a very flexible and fast confocal system build with a leica dmi 6000 cs inverted, fully automated microscope with motorized stage, condenser, objective and filter turrets. The leica tcs sp5 mp covers a wide range of imaging applica tions multiphoton and one photon by combining two technolo gies in one system.
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